Studies of Single-Chain Antibody Expression in Quiescent Escherichia coli
نویسندگان
چکیده
منابع مشابه
Studies of single-chain antibody expression in quiescent Escherichia coli.
Quiescent Escherichia coli cells are generated by overexpressing the Rcd transcript in an hns-205 mutant host. The resulting nongrowing, metabolically active cells were used here to express a single-chain antibody fragment (scFv) in shake flask and fermentor cultures. The expression system is based on two plasmids; one carries the product gene expressed from lambdaP(L) under the control of the ...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2004
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.70.5.3005-3012.2004